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  • Bryan Johnson
    Bryan Johnson @bryan_johnson

    5/ Slowing Cognitive Decline in Mild-to-Moderate Alzheimer's Disease:

    A randomized placebo-controlled trial with 321 participants with mild-to-moderate Alzheimer's disease found that 138 mg/day of methylene blue:

    + Improved performance on the Alzheimer's Disease Assessment

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    Aug 11, 2025 2 0 View on X
  • Bryan Johnson
    Bryan Johnson @bryan_johnson

    6/ Improved mitochondrial function and cellular respiration during exercise in dogs

    An older study from 1986 investigated the effects of methylene blue on dogs during exercise.

    Protocol: Methylene blue was infused directly into the left atrium of the heart, starting with a

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    Aug 11, 2025 1 0 View on X
  • Bryan Johnson
    Bryan Johnson @bryan_johnson

    3/ Cognitive enhancement, and reversal of neurodegenerative disease

    Long-term methylene blue treatment in mouse models of Alzheimer's and Parkinson's disease has shown promise, not only by reducing pathological protein aggregates (Aβ plaques and phospho-tau tangles) but also by

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    Aug 11, 2025 2 0 View on X
  • Bryan Johnson
    Bryan Johnson @bryan_johnson

    4/ Methylene blue shows cognitive enhancement effects, slows down neurodegenerative disease, and improves mental health in PTSD patients.

    Cognitive Enhancement in Healthy Adults: A landmark randomized controlled trial (RCT) in healthy volunteers demonstrated that a single low

    Aug 11, 2025 1 0 View on X
  • Bryan Johnson
    Bryan Johnson @bryan_johnson

    0/ Methylene blue health effects are not yet well-researched, especially as it relates to fitness, performance, and longevity potential.

    But it’s intriguing for mitochondrial function and energy enhancement, particularly for brain health in the context of neurodegenerative

    Aug 11, 2025 1 0 View on X
  • Bryan Johnson
    Bryan Johnson @bryan_johnson

    1/ My methylene blue protocol

    + 25 mg/day of methylene blue orally
    + 5 days a week
    + Keeping a 2-day weekly clearance break to prevent accumulation, especially in the brain

    Aug 11, 2025 3 0 View on X
  • Bryan Johnson
    Bryan Johnson @bryan_johnson

    2/ What I will be measuring

    Mitochondrial health and function (complex activity and functional assessment)

    + Exercise performance: VO2Max and muscle strength, lactate/pyruvate ratio during exercise
    + Brain health and function: blood biomarkers (pTAU217, S-100B) and Kernel-flow

    Aug 11, 2025 1 0 View on X
  • Bryan Johnson
    Bryan Johnson @bryan_johnson

    I’m experimenting with methylene blue.

    Clinical trials in humans show promise for brain health.
    Animal studies show minimal to no efficacy in lifespan extension.

    The potential: mitochondrial function and energy. 🧵

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    Aug 11, 2025 84 3 View on X
  • Bryan Johnson
    Bryan Johnson @bryan_johnson

    this is my sleep data and morning workout

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    Aug 11, 2025 34 0 View on X
  • Bryan Johnson
    Bryan Johnson @bryan_johnson

    guys I went to bed late last night and everything is fine this morning. maybe i’ve taken this longevity thing too far?

    Aug 11, 2025 897 17 View on X
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