@CatallaxySpirit We need to measure something to assess the success of interventions. We use biomarkers as a surrogate for longevity and disease. This is true throughout clinic medicine. Any clinic or physician would do the same, as there is no other option for individual care.


@verviticus Braintree, Kernel and Blueprint all have/had the same goals:
1. Customers write us love letters.
2. Team member say it's the best job they've had.
3. We're best-in-class.

I work very hard to build enduring culture and enable the best talent. Many more very happy employees…


@notVitaliy I’ve experimented with many peptides over the years including BPC 157, Cerebrolysin most and recently and I use topical peptides on my hair and skin now. They’re effective when used precisely. However, there is limited research for many peptides, so it is hard to make a blanket…


@iallarak For hair loss issues topical application of finasteride is safer (due to minimal systemic absorption) and comparably effective to oral dosing.


@tim12197355 Resveratrol doesn't meet the evidence criteria to be included in the stack. We’re open to adjusting if that changes in the future.


@_julian256_ Eggs are great and have a good macro/micro nutrient profile


@DoctorPerin Love and deep social bonds can reduce mortality risk by up to 91%. A landmark meta-analysis with 309k participants found meaningful and complex social relationships reduced mortality risk significantly.

For the first 34 years of my life, I believed the opposite narrative: to be…


@ricfranco Optimize for health by lowering your RHR (resting heart rate) before bed.  It will do more for your health than anything else.

+ it will help you create great sleep habits
+ sleep quality determines will power and if you'll exercise
+ exercise and will power determine if you…


@AstonScott1 I think we want to be what we want AI to become. When/if AI becomes sentient, it may assess how we humans treated other forms of intelligent life and form conclusions about our character.


@luishxyz Yes this is about personal health, but really, it's about turning humanity's attention to realize that we are the first generation who won't die. I'm after a philosophical revolution and a reimagination of intelligent life.


@j4nve If money were my goal, I'd be doing something else. I'm investing my money into a mission to redefine what it means to be human and the potential of intelligent existence.


@ex_deus6 Just started. Will share more soon.


@EthanBlake_LTL We've built an anti-grey formulation - early signs show SCF, VEGF, bFGF growth factors reawaken pigment cells + improve microcirculation. I've seen good results.


@samurai1269 Optimize your diet for blood sugar control. Focus on protein, low glycemic foods, remove added sugars and refined carbs, you can also try a lower carb diet or Mediterranean-style diet. All Blueprint products are on point with these recommendations. That in combination with…


@extraharsh From a regulatory standpoint, it’s difficult for supplement companies to publish research linking their products to medical outcomes because doing so can reclassify the supplement as a drug under FDA rules. This would require extensive clinical trials and FDA approval, which are…


@marijnvanduuren we've upgrading the mac nuts to glass. Will ship in the coming months. Generally speaking, existing packaging options are terrible. We've been working with brand new non-plastic material companies and hope to have a new line out in the fall.


@AIMemeCreator in a world of superintelligence, it's not clear that the human race has a path forward.


@drstevebool thank you anthonny


@ASTRASMYTH that I'm doing this to make money, I don't need it.


@GerardAlba thank you friend


@Jon_The_Hacker we need humanity to have a fervent desire to honor existence as the greatest virtue and focus all our energy and money towards that


@the_Arow_H yes, gene therapy will play a critical role


@cogentia more coming soon


@artiscode I’ll give you the top 3:

1) Sleep 7-8 hours per night
2) Exercise to improve your VO2 max and cardiovascular  fitness
3) Eat this:


@jhaverinator We’re launching a Don't Die restaurant certification soon.


@NickSchmidt_ Would love for you to attend one. I'm hoping to train up others too soon so that we can begin hosting them in intimate settings around the world. It takes two hours of dedicated conversation to understand Don't Die.


@WaxlNakamoto When death is inevitable, everyone chooses their favorite YOLO game. When death is no longer inevitable, existence becomes the highest virtue.


@HonorTheCall June: hair
August: skin


A lot of energy pointed my way lately…hate, misinformation, and helpful critique.

AMA 👇

Context:
+ I am suggesting that we are the first generation who won’t die.
+ I am offering that existence is the highest virtue.
+ We are giving birth to superintelligence.
+ We are…


@TigerWoods you sleep in a hyperbaric chamber too?


who saw that coming?


@pepeDRT bring out your night owl, the possums predator


@nikmarino9 any new interesting longevity therapies?


@BillyM2k bet that feels amazing


@Tom_Whitehouse1 painful, rooting for you. get that baby on a sleep protocol.


@jasongoodman__ yup I hear you, dump the tortilla and enjoy


@HendoAi not in a while, we have so many exciting things happening


@DextMoon use responsibly


@JohnWRichKid in that case wendy's is eating you


How's everyone doing...

going to bed on time and exercising daily?


@demishassabis @IsomorphicLabs Congratulations Demis, I love seeing AI applied to solving disease


@theliverdr such poor taste and etiquette publicly posting private messages


@karpathy Andrej this is an excellent write up and is consistent with my experience. Very happy to hear that you're thriving with high quality sleep.

Interesting to learn of your RHR and sleep relationship. I've been meaning to look into this more. A quick deep research offers:

•…


This is an excellent write up by @karpathy .

"Yes, sleep matters. Overall, I will say with absolute certainty that Bryan is basically right, and my sleep scores correlate strongly with the quality of work I am able to do that day. When my score is low, I lack agency, I lack…


@theliverdr Cyriac why are you so angry? Who hurt you?

Blueprint offers extra virgin olive oil, proteins, nuts, and nutrients which have independent and robust scientific evidence. They are third party tested. The certificates of analysis are publicly available. They are affordably priced.


@PSkinnerTech @WHOOP yeah red eye flights are really terrible; they cost more time than they save. good thing is that you've learned.


@nicknorwitz Nick was great to catch up with you.


Late night cravings hit hardest when willpower’s low. Decide now when you’ll eat your last meal today.


No prize in this moment is worth sacrificing the future.


Our enemy is our proclivity to YOLO our way to death.


@meltingdiodes the majority of the CoAs are up


Bryan Johnson hate is good for business.


credit @Heavensshiranui


@GratefulApe_eth U.S. for now though Europe is on the roadmap


Founders - come build Blueprint Labs into a billion dollar business with me.

Blood panels, speed of aging, organ biological age, MRI and more.

Typeform 👇


@balazskegl @drmichaellevin Cool conversation. Love this way of thinking about life.


@omarkassamX Yeah it's a known issue. We proactively emailed everyone who purchased them to explain the situation and offer to replace.


Blueprint customers - we're all caught up post the Netflix doc.

Status:
+ 4.75/5 customer satisfaction score
+ 1 day response times. Receiving love letters from you is our #1 goal.


The 2nd X Chromosome rescues the day for the aging female brain

Published earlier this month, a study demonstrated that the reactivation of the second X chromosome improves brain function in the brains of aging female mice

Background
1/ Females have XX chromosomes while males…


Do you trust your own thoughts?


@jurgenwriter Yeah, as her response to you shows, she easily becomes explosively violent. It's known as reactive aggression, where someone reacts impulsively and violently to a perceived threat or provocation, feeling justified in their behavior. To preserve her self-image, she engages in…


Pull this card and you’re basically immortal


@Rainmaker1973 the ultimate excuse for all things


@AutismCapital not a great team player


@justincgohn sadness that there’s no feeling


@cremieuxrecueil also seems boring


Women are four times more likely to develop autoimmune diseases, linked to having two X chromosomes.

These findings will enable better screening and earlier detection of autoimmune risk in women.

Background
1/ Autoimmune diseases affect up to 50 million Americans, 4 out of…


@DementiaLeague The meta-analysis covered 55 studies, and is transparent about a few studies, like the one you cited reaching opposite conclusions. Important to remember that we are dealign with observational evidence here, where sample size also reflects on the  statistical strength of this…


Statins reduce dementia risk by up to 63%

Published earlier this year, the largest meta-analysis to date involved over 7 million participants in 55 studies and concluded that statin use reduced the risk of Alzheimer Disease and Dementia.

0/ Being exposed to statins for over 3…


@hubermanlab @doctorstaci @RobertKennedyJr @esaagar @ScienceMagazine @joerogan and here is a scientific breakdown of fluoride in drinking water


What's your opinion on death?


[1]
[2]


If you're going to get a tattoo, here's how to reduce risk:

1. Limit the tattoo size to 2"x2".
2. Use tested and approved tattoo inks [1] [2]


Tattoos, especially larger ones, increase your skin and lymph nodes cancer risk. This is how you can mitigate the risk.

0/ A recent study on Danish twins found that having a tattoo at all (exposure to tattoo ink) increased the risk of skin cancer and lymphoma by 33%, while a…


@techno0ptimist My goal is a 10 million x return on investment, getting humanity to spend 100 trillion annually on don’t die


@brian_armstrong @newlimit Brian I’m very excited about what you’re building. One of the most exciting things happening in longevity.


@GuruAnaerobic A mile time of 5.20 at 63/64yrs would be insane, rooting for you


Building life systems will help you make decisions your best self wants and your worst self would otherwise  sabotage.


@platinummolar that's exactly right, the research shows it restores lost function but doesn’t enhance normal function.


@MacroMate8 keep at it friend


@darthur there are so many to choose from now and they're all good enough to help create intuitions on lifestyle


@arcorren great work, I'm sure you feel great


@EvanMoyle REHIIT is also good and operates via different mechanisms. Norwegian 4x4 has more bust evidence.


aka Norwegian 4x4


@josephluria there's good clinical evidence that 4 min pushes the cardiovascular system into a high-effort zone long enough to maximize VO₂ max and heart health adaptations and being sustainable across multiple rounds.


@Majiq_Mat That's right, 4 min of high exertion and 4 min of rest, cycled 4 times.


@realangrypom Lately I've been able to sleep through the entire night without getting up to pee about 50% of the time. I discontinue fluids in the late afternoon which has helped.


@iashwinikumaar it's euphoric, I really look forward to HIIT


@ulises_cy yes I do HIIT 3x a week which is on the upper end. 1-2x  a week is a good target. Like all things, not too little and not too much.


@businessbarista Your target would be: 150–169 bpm
Calculated by:
1. HRmax = 220 - your age
2. then calculate 80-90%


High intensity interval training (HITT) is one of the best things you can do for your health. Get 1x in this week.

Warm up
4 min at 80-90% your max
4 min rest, keep moving
Complete 4 rounds of this (4x4)

Can be done via elliptical, running, cycling, rowing...


@nikitabier I've got you Nikita


@AgentChud your penis is safe with me


@Lifelessrealm lol twitter is my diary


@Abe_Froman_SKC who knew an ED drug might end up being a longevity drug


@CYBER__BULLY 🫡


@nic__carter I didn't do a great job explaining with that last post. Here's the full context.


[1]
[2]
[3]
[4]
[5]


@kevinf0ught I encourage people to follow scientific evidence and measure their biomarkers.


“I thought your erections were natural”.

I didn’t do a great job on that last post. Now this topic is trending… let me try again.

My erections improved by 34%, independent of Tadalafil.

FYI, I was always transparent about taking Tadalafil. When I first started talking about…


@levelsio 100%, exactly what I see. We have mistakenly built a culture of Die.


yes, this is my mom


when you have a rave at 7 but bedtime at 8


@aravind thank you for the advice, I choose to tempt fate in the quest to end death


gm friends


@nic__carter the night time erection data is legitimate. Read the thread, it has the science. Cialis is not responsible for my nighttime erections because studies show it has no significant effect on nocturnal erection duration in healthy men; its primary benefit is in restoring normal…


@boneGPT Read the thread. Cialis is not responsible for my nighttime erections because studies show it has no significant effect on nocturnal erection duration in healthy men; its primary benefit is in restoring normal erectile function in those with ED.


7/ Never mix Cialis (or any ED pill) with nitrate heart meds (e.g. nitroglycerin)

That combo can cause a dangerous blood pressure crash. Also be careful if you're on multiple BP meds or have very low BP. Always consult your doctor first.

A study comparing 55,777 men treated…


5/ Cialis can boost your brain

Better blood flow might aid cognitive functions too, especially as we get older.

In a small trial, older men taking daily Cialis improved on cognitive tests of attention and processing speed. Even their brainwave patterns (EEG) got sharper,…


6/ Long term Cialis use is generally safe in men, but comes with some possible side effects

Tadalafil is a prescription drug. Common side effects at low doses: headache, flushing, nasal congestion, or heartburn – usually mild. It's considered safe for long-term use in many men…


3/ Cialis can improve your metabolism, blood sugar control and can help increase fat burning.

Daily Cialis might improve how our bodies handle sugar. In a 2022 trial with diabetic men, 5 mg/day modestly lowered HbA1c (blood sugar marker) over 6 months, Suggesting better insulin…


4/ Cialis reduced chronic inflammation, a major driver of aging

Termed “inflammaging”, chronic inflammation speeds aging. In a small study, men on daily Cialis for 6 months saw their elevated inflammatory markers (e.g. IL-6) drop back to normalA calmer immune system = a…


1/ My nighttime erections are better than the average 18 year old, but Cialis is unlikely to play a meaningful role in them or even my sexual function.

While it’s true that daily low-dose Cialis (2.5–5 mg) can help men with erectile dysfunction (ED) or benign prostatic…


2/ Here is why you may consider taking Cialis

Cialis’s effects on blood vessels might benefit many organs. It boosts nitric oxide (NO), relaxing arteries.

Enabling better circulation, organ blood flow, and a bit lower blood pressure. Basically helping to keep your blood…


I take 2.5 mg of Cialis daily.
Not for erections, but for longevity.

Here’s what the science says about Cialis and lifespan extension…

And why it’s not the reason behind my 3 hour long titanic nighttime erections.
🧵


0/ It sounds wild, but low-dose Cialis (tadalafil) and Viagra (sildenafil) are both drawing attention for longevity benefits.

Recent clinical evidence indicates the use of either correlates to longer survival in men, in addition to their potential for heart health, metabolism,…


@GuruAnaerobic @AbudBakri most people don't realize that getting high quality sleep takes a lot of hard work and life systems


@FedericoNoemie you have good taste in co-workers


@TurnerNovak was great hanging Turner


@AbudBakri No, I do not take clomid/enclomiphene/Gonadarelin


@kcryptoinvest welcome friend, we're going places never seen before


@LibertarianLars It's funny that the NYT is trying to vilify consent


If the headline were written for accuracy, it would say:

"Bryan Johnson made sure employees were consensually agreeing to their working environment"


Applications to work with me have increased.


@NayakSatya_SG Yeah it's pretty cool following the scientific evidence and measuring extensively.


@AdnanBoz My daily protein comes from Blueprint proteins (hemp and pea), collagen peptides, and legumes; smaller amounts of protein in berries, nuts, seeds and vegetables.


@VincenzoPala That’s a common misconception. Thyroid medications such as levothyroxine and Armour Thyroid are prescribed to normalize thyroid hormone levels, not to elevate FSH. FSH is regulated by the hypothalamic-pituitary-gonadal (HPG) axis, while thyroid hormones operate through the…


@yslburnerr Years ago, we ran a caloric restriction longevity experiment, testing a 25% caloric deficit (1,950 kcal/day). Knowing this level of restriction could potentially lower testosterone, we proactively introduced TRT, not in response to a measured drop, but as a precaution.

After a…


I am 47 and have ideal levels of testosterone (735 ng/dL). I am not on TRT.

My LH (9.7 IU/L) and FSH (7.4 mIU/ml) are also ideal. Evidence that I am not on TRT.

These testosterone, LH and FSH measurements were taken 60 days ago, in Jan of 2025.

My protocol:

+ I consume…


To answer other questions:

1. We published the results of all participants that completed the entire study (results below).

2. Regarding the sharing of raw data, there are regulatory constraints. We have shared the results in a way that accurately communicates results,…


@R89Capital I’ll add that we face regulatory constraints and have shared the results in a way that accurately communicates results, maintains scientific integrity and remains within regulatory boundaries.


@R89Capital We published the results of all participants that completed the entire study.


@R89Capital Results were emailed to study participants and also shared here:


@liltestmoretren People on Blueprint are carnivores, omnivores, keto, paleo and plant based. Everyone is welcome.


Within weeks of the study starting, we identified the sources of side effects and implemented targeted product updates:

#1 Reduced the amount of allulose, a functional sweetener that also acts as a health-active potentially supporting weight management, fat oxidation, and…


The New York Times knowingly published false and misleading statements about Blueprint:

“Participants…apparently saw their testosterone levels drop, became prediabetic, and reported side-effects including nausea and bloating…” and claimed Dr. Zolman departed over the study…


So @EuroValentino
1. posts something libelous about me
2. a bunch of unthinking people pile on
3. I call him out on the ridiculousness
4. he ducks away making the post unviewable after the damage is done


@foxenflask 🤝


@EuroValentino This is ridiculous and you made it up.

There is no credible evidence, clinical or mechanistic, that a nutrient-dense, evidence-based diet like the Blueprint protocol causes hypothyroidism.

“nuts, grains, almond milk, and undercooked vegetables” bears no resemblance to the…


@MarioNawfal The absurdity of their hit piece makes one wonder who is behind the scenes puppeting them.


@skooookum easy to reconcile, just wait to smoke the cigarettes until 2100 when we’ve figured out how to make smoking into a longevity drug


Miami Don’t Die Summit is today!


@hegesia_c The NYT reporter “fact checked” this question and below is what I shared, but she entirely ignored the facts as it conflicted with her take down narrative:

“The decision to highlight the 5.1 year reduction was made entirely by the documentary’s production team. I did not…


RT @rivatez: Lol, this NYT piece isn't even a story. At most, it shows @bryan_johnson  is prudent about legal matters, as any successful pe…


The NYT just used my ex as their star witness in trying to take me down. Turns out that both she and the NYT have credibility problems.


I’m the asshole who left his ex when she got cancer. Right?

Wrong.

Here are the receipts.


@benjileibo basically


@boneGPT their hatred of me has been increasing in proportion to my success in slowing my speed of aging


@noxlonga it’s wild when you know the facts and then read an account that creates a different reality


@solanapoet this article, ironically, explains why opt in confidentiality agreements are a fair and good practice for everyone


@chasedownleads thanks, turns out the NYT is flaccid


The New York Times tried to come for me and missed.

What was meant to be a takedown ended up reading like a profile piece.
+ they spent a year digging
+ talked to 30 people, incl former fired employees
+ propped up my ex as their star witness (who tried to extract $9 million…


@Mind_Essentials you actually can add days to your life


@LetMeChatGPThat @GillVerd tough one, I think HRV as peace is the most scarce of resource in a relationship


@eimisja respect for the follow through


@TiffanyFong_ tiffany you celebrated your bday with death and I invite you to celebrate with life next year


@prmshra parm I know you know better and penance you to 30 minutes of cardio


@rsg be careful as zyn will own you and you will then be its servant


Smart people are dumb without sleep.


@ElonMuskAOC I feel forgotten


@shrinath_prabhu best to act early and fast, I have a video on my YT channel


@cz_binance I’ve heard that crypto really knocks you out 😂


@TheAlgoholic Haha, nothing exciting. Washing my face, brushing my teeth and talking to my son.


@HungryPawnsX The worst, and so hard to know which one is beeping


@RealRadDad yeah I hear you, have you tried creating a family culture of sleep, creating rules and star charts?


@Shiva_SPC great job


@nordin_eth bathroom, I had a busy morning and didn’t hydrate properly and caught up I the afternoon which resulted in 1x bathroom wake up.


@toplonging be careful that it doesn’t backfire


My soul is at peace with reality.

My head hits the pillow and I’m out within minutes. My nervous system is steady calm all night.


I receive messages like this from friends all the time. This one from a hard-charging AI entrepreneur.

Trust me, making sleep your #1 priority is the best thing you can do in life.


@koharanmol @noahkagan I had no money and in fact had intense financial pressures from building multiple startups while having babies.


@noahkagan I have three children and am an extremely attentive father. New borns are unquestionably challenging. I wish I’d had a positive influence in my life nudging me and the entire family towards better health habits during those challenging times.


@GN0ME0GN0ME0 I  think possibly closer to an Elf or Vampire however I'm open to a Wizard arc


@jonkalis and how do you feel now about those decisions?


@NorthShoreHaole seems you're half way there on the path to freedom, just on the outer edge


@the_bentist the universe would not be true if this were not the case 😂; your honesty is appreciated and respected. it's possible those who admire you will find strength in learning from your resolve to kick the habit.


@eimisja please report back completion


@jaxonparrott despite our best efforts, this variability will occur. that you know and are aware is great.


@michaeltlasley sometimes in pinch, suboptimal trade offs need to be made. a good question to ask is whether the decision was necessary.


Confess your debauchery


7/ 466 drugs were identified that can counteract the effects of 29 genes out of the the 64 genes identified to drive brain aging. Of these 29 drugs have shown some effect in clinical trials in counteracting brain aging, and 20 are considered geroprotectors (anti aging drugs).…


Significance

8/ These results demonstrate the viability of a dual structural and genetic approach to measuring brain aging and identifying targets that can slow it down. The study managed to independently validate various genetic pathways and drugs with known clinical efficacy…


9/ Drug repurposing is an attractive strategy to identify promising tools against aging and age related disease, at a fraction of the cost and effort required to discover and develop entirely new therapeutic agents.


4/ The analysis of BAG scores against gene expression patterns of participants blood and brain tissues identified genes that are likely to drive brain aging.


5/ 64 genes were identified with causal relationships to brain aging, of which 37 and 7 genes were validated as having two and three pieces of genetic evidence supporting their effect, respectively.


6/ The identified key genes also have associations with phenotypes and conditions that are linked to accelerated brain aging including: increased BMI, high blood pressure, smoking, asthma, Alzheimer and Parkinson disease.

Some specific examples
+ MAPT correlated with increased…


0/ Published last week in @ScienceAdvances a study reported the use of brain scans with multiple deep learning models combined with gene expression analysis in blood samples and brain tissues from  to identify genes that drive brain aging.


Background and details

1/ The study used brain scans and gene expression analysis data from 38,961 subjects from the UK Biobank, 6637 of which with a diagnosed brain disorders with “healthy” brains.


2/ The study aimed to match structural (MRI) and genetic insights into brain aging to guide drug repurposing for delaying and slowing down brain aging and related disorders.


3/ The analysis of brain MRI scans using 7 deep learning models yields an estimation of the biological age of the brain,  Brain Age Gap BAG, the estimated gap between chronological and brain biological age.


Study uses MRI ad machine learning to estimate brain age, and identifies 64 “druggable” genes that drive brain aging


5/ I'll be posting soon about what you can do to  improve sperm health.


4/ Abnormal sperm parameters correlate with a shorter life expectancy

A study involving 11,935 men evaluated for infertility found that men with abnormal sperm parameters had higher mortality rates. Men with two or more abnormal sperm parameters had a 2.3 fold increase in all…


2/ Poor sperm counts predict lower testosterone levels and poor metabolic health.

A study involving 5177 found that men with a total sperm count of below 39 million per ejaculate were 12 times more likely to have abnormally low testosterone levels. Moreover, men with low sperm…


3/ Poor sperm quality predicts higher risk of age-related chronic disease and addiction

A study comparing 13,027 men with semen-related infertility to 23,860 men with no semen abnormalities showed that men with abnormal semen parameters (male factor infertility) had 30% and 48%…


0/ I have more motile sperm than the average healthy 22-29 year olds and much higher than the average fertile US man (104 million motile sperm count for the US father, age 32∓6 years).

Image above of my sperm is from a 2023 test.


1/ Sperm quality predicts health and longevity

Beyond predicting infertility, poor sperm quality also correlates with poorer health, higher incidence of age related disease, and premature death. While a total motile sperm of 20 million is enough for fertility, this number is a…


My sperm quality equal to a healthy 20-year-old.

+ sperm 330 million
+ motility 53%
+ motile sperm 175 million
+ morphology 10% normal

Sperm health predicts testosterone, metabolic health, disease, addiction, and life expectancy.

Here’s what you need to know.🧵


@TiffanyFong_ @jacqmelinek hbday...try celebrating w tea as alcohol worsens skin health by dehydrating and weakening skin barrier leading to dryness, dullness, and increased fine lines.

Also triggers inflammation and oxidative stress, accelerating collagen breakdown and premature aging. Also disrupts gut…


Many people are depressed...and many depression treatments don't work.

Theres a new treatment protocol that has a 78% success rate. It only takes 5 days and worked in treatment-resistant patients. It's called SAINT TMS.

My cofounder @_katetolo  was depressed and tried it.


@boneGPT my system was  ~$100,000 and is in my garage making it very easy to do it daily. Also, the 21% oxygen in the chamber allowed me to work during the session. I'm guessing many more health clinics will be getting systems like I have making it easier for you to do it close to home.


@ichorid805 hopefully more providers in the market will make it lower cost for everyone


@slotkinjr I'm similarly curious. Current plan is to follow up the 60 initial sessions with 20 sessions every 90 days however there is limited evidence to support this is the ideal protocol. We will continue to measure and assess.


I used a @hpotech_medical chamber


6/ My HBOT protocol in a glance

+ Hard shell chamber from @hpotech_medical
+ 2 ATA
+ 90 minute sessions
+ 20 min breathing 100% oxygen. 5 min break. Repeat.
+ 60 sessions within 90 days.

The protocol is consistent with the most credible clinical studies up to date.

I…


7/ Limitations

While doing HBOT I also underwent three total plasma exchanges (TPE).


8/ Don't Die Certified Clinics - coming soon

Wanting to do HBOT and but don't know where?

Soon, you will be able to find a clinics and health practitioners that are Don't Die Certified, meaning they follow the same scientific rigor and measurement as my protocol.

We have…


4/ Brain health

28.6% reduction (from 0.14 to 0.1 pg/mL) in the brain damage and dementia marker pTAU127.

The phosphoprotein p-TAU217 is a specific marker for Alzheimer disease, with levels higher than 0.18pg/mL potentially indicative of plaque accumulation in the brain, hence…


5/ Skin

+ my facial skin got 1 year younger (Pre-HBOT: 39 years→ Post-HBOT: 38 years)
+ major improvement in UV spots score  (55th→ 68th percentile)

Indicative of significant removal of Advanced Glycation End-products AGEs from the skin, ostensibly due to improved…


3/ Microbiome

Dramatic improvements in my microbiome:

+ Akkermansia muciniphila increased by 1,000%.
Pre-HBOT: Not detectable → Post-HBOT: 4.2E4 CFU/g

Akkermansia is linked to gut barrier integrity and metabolic health.

Note, I started Akkermansia on August 9th and two…


1/ Vascularization

300% increase in VEGF drives the formation of new blood vessels which improves tissue perfusion and oxidation, essential for improving fitness, physical and cognitive performance . Also has further pro-longevity effects on gene expression.

Improved muscle…


2/ Telomeres

Telomerase activity of a 12 year-old (7.7%). Telomers keep chromosomes from falling apart. They are essential for healthy cell division, including stem cell renewal. As we age, cells lose their telomerase activity and telomeres get shorter, when telomeres are too…


HBOT ranks as one of the highest value health therapies I’ve done. Results from 60 sessions:

+ wiped out all systematic inflammation in my body.  Below detectable levels. This is wild.
+ 300% increase in VEGF (formation of new blood vessels)
+ telomerase activity of a 12 year…


0/ Inflammation

Post HBOT, there’s no detectable systemic inflammation in my body (hsCRP). Inflammation is the foundation of disease and all things bad health.  Before HBOT, my inflammation markers were in the top 5% of 18-29 year olds (you get more inflamed as you age).

HBOT…


@Tonyyjabroni two bananas before bed will raise your resting heart rate by 5-10 bpm, delay sleep onset by 15-30 minutes, result in a mid-night awakening due to the glucose crash, and you'll lose about 10% sleep efficiency. So you won't die but you won't feel rested in the am.


@danestephyoo it's always me posting


I see what you're up to
stop scrolling
put the food away
get off the couch
go to bed


@astralmatrix proud of you


@kennandavison hey I know that guy


gonna be such an exciting week
+ hbot results
+ NYT hit piece drops
+ sperm results
+ sold out Miami don't die summit
+ don't die dinner with zac efron
+ don't die certified launch
+ seeing my mom on Thursday


this hurt


The coolest thing about this beautiful Monday morning is that we are alive.


@arthurcolle you have acknowledged and confessed, go forth with strength and build systems that enable AI agents to flourish


@Jiankui_He how bout vampires


@NewsyJohnson i’ll pass it back to you if you post perfect sleep scores for a week


@mhiggins remarkable how fast it's taken hold


Result coming up this week:
1. sperm health
2. HBOT results


@TiffanyFong_ urgently slow your speed of aging so that Tuesday never arrives


@jiratickets 😂 great write up and storytelling


I thought I was fairly measured and calm 😂


Spend the day reading and not scrolling.


Newsy Johnson out
Bryan Johnson in


Weekends are for renewal, not ruin.


@nonfungible_jan matcha it sold out really fast, back in stock very soon


@KevinJWelti yes, Kevin, I'd love to have you over sometime for dinner


@Kwebbelkop Here's a video on how to prepare it. Add 2 g of Matcha.


@KevinNaughtonJr there's a few good options if you search amazon for food-grade 18/8 stainless steel


RT @battleangelviv: i’ve been adopting many of @bryan_johnson’s basic recommendations and i regret to inform you that my life has, in fact,…


Matcha Nutty Pudding slaps


@McJuggerNuggets that’s a fun challenge


gtfo pls


@stats_feed Early days of my experiment I consumed 3 oz of red wine daily in the morning however stopped bc every calorie has to fight for its life and there are higher health-value things to consume.


Sleep awakens the soul


Alcohol makes you sad.


@Jason they asked for the data to assess impact


@nntaleb Nassim I’m a perfect friend for you


no words, I'm devastated


At a hotel in Austin burning incense in the lobby. Told the staff it's bad for their health and gave data to share with their manager:

Toxin increase:
. PM 2.5 by 385% (= to 6 cigarettes)
. PM 10 by 408%
. PM 1.0 by 453%

Left (outside), right (lobby).
Don't burn incense.